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Isolation and partial characterization of bacteria (Pseudoalteromonas sp.) with potential antibacterial activity from a marine costal environment from New Caledonia ArchiMer
Dufourcq, R.; Chalkiadakis, Eletherios; Fauchon, M.; Deslandes, Eric; Kerjean, V.; Chanteau, S.; Petit, Elodie; Guezennec, Jean; Dupont-rouzeyrol, M..
Marine bacteria are a rich source of bioactive metabolites. However, the microbial diversity of marine ecosystem still needs to be explored. The aim of this study was to isolate and characterize bacteria with antimicrobial activities from various marine coastal environment of New Caledonia. We obtained 493 marine isolates from various environments and samples of which 63 (12.8%) presented an antibacterial activity against a panel of reference pathogenic strains (Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis). Ten out of the most promising strains were cultured, fractionated and screened for antibacterial activity. Four of them (NC282, NC412, NC272 and NC120) showed at least an activity against reference and...
Tipo: Text Palavras-chave: Antibacterial activity; Characterization; Marine bacteria; New Caledonia; Screening.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00174/28552/26994.pdf
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Partial characterization of an exopolysaccharide secreted by a marine bacterium, Vibrio neocaledonicus sp nov., from New Caledonia ArchiMer
Chalkiadakis, Eletherios; Dufourcq, R.; Schmitt, Sophie; Brandily, Christophe; Kervarec, N.; Coatanea, Denis; Amir, H.; Loubersac, Lionel; Chanteau, S.; Guezennec, Jean; Dupont-rouzeyrol, M.; Simon-colin, Christelle.
Aims Exopolysaccharides (EPS) are industrially valuable molecules with numerous useful properties. This study describes the techniques used for the identification of a novel Vibrio bacterium and preliminary characterization of its EPS. Methods and Results Bioprospection in marine intertidal areas of New Caledonia followed by screening for EPS producing brought to selection of the isolate NC470. Phylogenetic analysis (biochemical tests, gene sequencing and DNADNA relatedness) permitted to identify NC470 as a new member of the Vibrio genus. The EPS was produced in batch fermentation, purified using the ultrafiltration process and analysed by colorimetry, Fourier Transform Infrared spectroscopy, gas chromatography, Nuclear Magnetic Resonance and HPLC-size...
Tipo: Text Palavras-chave: Biopolymers; Biotechnology; Exopolysaccharide; Fermentation biotechnology.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00144/25501/24036.pdf
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Quelques aspects bioécologiques de Gamberdiscus toxicus, peridinien benthique responsable de la ciguareta à Tahiti ArchiMer
Bagnis, R.; Inoue, A.; Rongeras, S.; Galonnier, M.; Bennett, J.; Chanteau, S.; Pastural, J..
We have carried out on a fringing reef of Tahiti, catching periodically ciguateric fishes, a comparative dynamic survey of the population density of GAMBERDISCUS TOXICUS and some linked environmental factors, during a four year period. Half of the marine parameters, i.e. temperature, salinity, dissolved NO2, NO3, PO34, SiO2, have been systematically studied according to a weekly or a monthly rhythm ; the other parameters, i.e. pH, dissolved NH3, 02, C and B12 vitamin have been studied episodically. The local pluviometry for the two weeks preceeding the samplings has also been measured. The statis­tical analysis of the data did not allow us to observe any significant simple correlation between the physical or chemical parameters and the density of G,...
Tipo: Text Palavras-chave: Toxines naturelles; Ciguatera; Peridiniens; Récifs coralliens.
Ano: 1982 URL: https://archimer.ifremer.fr/doc/00306/41755/40966.pdf
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